Rhee S, Ebensperger C, Dembic Z, Pestka S
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854-5635, USA.
J Biol Chem. 1996 Nov 15;271(46):28947-52. doi: 10.1074/jbc.271.46.28947.
The gene for the second chain of the human interferon-gamma receptor was analyzed from cosmid DNA clones. The gene spans over 33 kilobases of DNA and contains seven exons. The signal peptide is encoded by exons 1 and 2, the extracellular domain by exons 2, 3, 4, 5, and by part of 6. Exon 6 also encodes the whole transmembrane domain and part of the intracellular domain. Exon 7 encodes the remainder of the intracellular domain and contains the 3'-untranslated region. The sequences at the exon/intron boundaries are well conserved with respect to canonical acceptor/donor sites (AG/GT). The 5'-flanking region was sequenced and analyzed for transcription factor binding sites. No TATA or CAAT boxes in the promoter region were identified. Consistent with the lack of a TATA box, analysis of the mRNAs by primer extension showed multiple transcription start sites. Promoter activity of the 5'-flanking region was investigated with a luciferase reporter gene and the cytomegalovirus minimal promoter. Segments of the 5' region with promoter activity were identified.
从黏粒DNA克隆中分析了人γ干扰素受体第二条链的基因。该基因跨越超过33千碱基对的DNA，包含7个外显子。信号肽由外显子1和2编码，细胞外结构域由外显子2、3、4、5以及6的一部分编码。外显子6还编码整个跨膜结构域和部分细胞内结构域。外显子7编码细胞内结构域的其余部分，并包含3'非翻译区。外显子/内含子边界处的序列在典型的受体/供体位点（AG/GT）方面高度保守。对5'侧翼区进行了测序，并分析了转录因子结合位点。在启动子区域未发现TATA或CAAT框。与缺乏TATA框一致，通过引物延伸对mRNA的分析显示有多个转录起始位点。用荧光素酶报告基因和巨细胞病毒最小启动子研究了5'侧翼区的启动子活性。鉴定出了具有启动子活性的5'区域片段。
