Ebensperger C, Rhee S, Muthukumaran G, Lembo D, Donnelly R, Pestka S, Dembic Z
F.Hoffmann-La Roche AG, Basel, Switzerland.
Scand J Immunol. 1996 Dec;44(6):599-606. doi: 10.1046/j.1365-3083.1996.d01-353.x.
A clone containing the gene ifngr2 for the second chain (IFN-gamma R2) of the mouse interferon gamma receptor complex was isolated from a cosmid library made of 129/Sv mouse genomic DNA. Sequence analysis revealed that the second chain is encoded by 7 exons. The complete gene spans about 17 kb of the genomic DNA. In the 5'-flanking region several transcription initiation sites between 27 and 136 nucleotides upstream from the translation initiation codon were mapped. This region has a high GC content, but no TATA or CAAT box. Potential binding sites were found for transcription factors Sp1, AP-2, NF1, EGR and NF kappa B. Promoter activity was assayed with a series of constructs with firefly luciferase as a reporter gene, under the control of the promoter fragments of various lengths. This region showed promoter activity in transiently transfected Chinese hamster ovary cells.
从小鼠129/Sv基因组DNA构建的黏粒文库中分离出一个克隆，该克隆含有小鼠干扰素γ受体复合物第二条链（IFN-γR2）的ifngr2基因。序列分析表明，第二条链由7个外显子编码。完整基因跨越约17kb的基因组DNA。在5'侧翼区域，翻译起始密码子上游27至136个核苷酸之间的几个转录起始位点被定位。该区域GC含量高，但没有TATA或CAAT框。发现了转录因子Sp1、AP-2、NF1、EGR和NF-κB的潜在结合位点。用一系列以萤火虫荧光素酶为报告基因的构建体，在不同长度启动子片段的控制下测定启动子活性。该区域在瞬时转染的中国仓鼠卵巢细胞中显示出启动子活性。
