Ivanov A, Vapalahti O, Lankinen H, Tkachenko E, Vaheri A, Niklasson B, Lundkvist A
Chumakov Institute of Poliomyelitis and Viral Encephalitides, Ac. Med. Sci., Moscow, Russia.
J Virol Methods. 1996 Oct;62(1):87-92. doi: 10.1016/0166-0934(96)02090-3.
A novel enzyme-linked immunosorbent assay, BLA IgM-ELISA, based on baculovirus-expressed Puumala (PUU) virus nucleocapsid protein, was developed for rapid diagnosis of nephropathia epidemica. The recombinant antigen (bac-PUU-N) was purified to homogeneity by HPLC and conjugated to biotin. The biotin-streptavidin system, in combination with the mu-capture technique, rendered the BLA IgM-ELISA a sensitivity similar to or higher than that of PUU virus IgM mu-capture ELISA based on native antigen. The assay was shown to be highly specific when evaluated using a panel of 160 patient and control sera.
基于杆状病毒表达的普马拉（PUU）病毒核衣壳蛋白，开发了一种新型酶联免疫吸附测定法，即BLA IgM - ELISA，用于肾综合征出血热的快速诊断。重组抗原（bac - PUU - N）通过高效液相色谱法纯化至同质，并与生物素偶联。生物素 - 链霉亲和素系统与μ捕获技术相结合，使BLA IgM - ELISA的灵敏度与基于天然抗原的PUU病毒IgM μ捕获ELISA相似或更高。使用160份患者和对照血清进行评估时，该测定法显示出高度特异性。
