Mercer J G, Hoggard N, Williams L M, Lawrence C B, Hannah L T, Morgan P J, Trayhurn P
Molecular Neuroendocrinology Group, Rowett Research Institute, Bucksburn, Aberdeen, UK.
J Neuroendocrinol. 1996 Oct;8(10):733-5. doi: 10.1046/j.1365-2826.1996.05161.x.
Leptin, the protein product of the adipose tissue-specific ob (obese) gene (1), reduces the body weight, adiposity and food intake of obese ob/ob mice on peripheral or central injection (2, 3, 4). [125I]leptin binding has been detected in mouse choroid plexus (5), from which a leptin receptor gene was expression cloned (5). The gene has at least 6 splice variants (6, 7). Leptin receptor mRNA was localized in the hypothalamus by in situ hybridization being particularly abundantly expressed in the arcuate nucleus (8). There is evidence linking the physiological effects of injected leptin with hypothalamic neuropeptide Y (9, 10) (NPY), which has potent central effects on food intake and energy balance (11), and is also expressed in the arcuate nucleus. Here we report dual in situ hybridization studies for leptin receptor and NPY gene expression in the mouse arcuate nucleus, where the majority of cells examined expressed both genes. This provides the first direct evidence that leptin acts on cells that express NPY mRNA.
瘦素是脂肪组织特异性ob（肥胖）基因（1）的蛋白质产物，在外周或中枢注射时可减轻肥胖的ob/ob小鼠的体重、肥胖程度和食物摄入量（2,3,4）。已在小鼠脉络丛中检测到[125I]瘦素结合（5），并从中表达克隆出一个瘦素受体基因（5）。该基因至少有6种剪接变体（6,7）。通过原位杂交将瘦素受体mRNA定位在下丘脑，其在弓状核中特别大量表达（8）。有证据表明，注射的瘦素的生理作用与下丘脑神经肽Y（9,10）（NPY）有关，NPY对食物摄入和能量平衡有强大的中枢作用（11），并且也在弓状核中表达。在此，我们报告了对小鼠弓状核中瘦素受体和NPY基因表达的双重原位杂交研究，其中大多数检测的细胞都表达这两种基因。这提供了首个直接证据，表明瘦素作用于表达NPY mRNA的细胞。
