Gasser R B, Bao-Zhen Q, Nansen P, Johansen M V, Bøgh H
Danish Centre for Experimental Parasitology, Frederiksberg C, Denmark.
Mol Cell Probes. 1996 Oct;10(5):353-8. doi: 10.1006/mcpr.1996.0048.
A random amplified polymorphic DNA (RAPD) technique using 16 decamer oligonucleotide primers was employed to characterize isolates of Schistosoma japonicum from seven geographical locations (Sj1: Zhejiang; Sj2: Anhui; Sj3: Jiangxi; Sj4: Hunan; Sj5: Hubei; Sj6: Sichuan; Sj7: Yunnan) of the People's Republic of China. Distinct differences between some isolates were reproducibly detected in RAPD patterns produced using five of the primers. The analyses showed that both Sj6 and Sj7 were quite distinct genetically from Sj1-Sj5 based on the presence/absence of particular bands (A10-200 bp, A9-220 bp, B17-520 bp, P205-680 bp and P235-930 bp). These findings are in line with previous reports on the biological, biochemical, immunological and chemotherapeutic differences of S. japonicum from Sichuan and Yunnan compared with other geographical regions. The present study showed, based on RAPD profiles, that genetic differences exist within S. japonicum from mainland China. This finding may have important implications for studying the population biology, epidemiology and clinical forms of the disease in China, as well as for developing vaccines and diagnostic test systems.
运用随机扩增多态性DNA（RAPD）技术，使用16种十聚体寡核苷酸引物对来自中华人民共和国七个地理区域（Sj1：浙江；Sj2：安徽；Sj3：江西；Sj4：湖南；Sj5：湖北；Sj6：四川；Sj7：云南）的日本血吸虫分离株进行特征分析。使用其中五种引物产生的RAPD图谱中可重复检测到一些分离株之间存在明显差异。分析表明，基于特定条带（A10 - 200 bp、A9 - 220 bp、B17 - 520 bp、P205 - 680 bp和P235 - 930 bp）的有无，Sj6和Sj7在遗传上与Sj1 - Sj5明显不同。这些发现与先前关于四川和云南的日本血吸虫与其他地理区域在生物学、生物化学、免疫学和化疗方面差异的报道一致。本研究基于RAPD图谱表明，中国大陆的日本血吸虫存在遗传差异。这一发现可能对研究中国该疾病的种群生物学、流行病学和临床类型，以及开发疫苗和诊断测试系统具有重要意义。
