Shrivastava Jaya, Qian Bao Zhen, Mcvean Gilean, Webster Joanne P
Peter Medawar Building for Pathogen Research, University of Oxford, South Parks Road, Oxford OX1 3SY, UK.
Mol Ecol. 2005 Mar;14(3):839-49. doi: 10.1111/j.1365-294X.2005.02443.x.
This study presents the first microsatellite investigation into the level of genetic variation among Schistosoma japonicum from different geographical origins. S. japonicum isolates were obtained from seven endemic provinces across mainland China: Zhejiang (Jiashan County), Anhui (Guichi County), Jiangxi (Yongxiu County), Hubei (Wuhan County), Hunan (Yueyang area), Sichuan 1 (Maoshan County), Sichuan 2 (Tianquan County), Yunnan (Dali County), and also one province in the Philippines (Sorsogon). DNA from 20 individuals from each origin were screened against 11 recently isolated and characterized S. japonicum microsatellites, and a set of nine loci were selected based on their polymorphic information content. High levels of polymorphism were obtained between and within population samples, with Chinese and Philippine strains appearing to follow different lineages, and with distinct branching between provinces. Moreover, across mainland China, genotype clustering appeared to be related to habitat type and/or intermediate host morph. These results highlight the suitability of microsatellites for population genetic studies of S. japonicum and suggest that there may be different strains of S. japonicum circulating in mainland China.
本研究首次对来自不同地理区域的日本血吸虫的遗传变异水平进行了微卫星调查。日本血吸虫分离株取自中国大陆七个流行省份：浙江（嘉善县）、安徽（贵池县）、江西（永修县）、湖北（武汉县）、湖南（岳阳地区）、四川1（茂山县）、四川2（天全县）、云南（大理县），以及菲律宾的一个省份（索索贡省）。从每个来源选取20个个体的DNA，用11个最近分离并鉴定的日本血吸虫微卫星进行筛选，并根据其多态信息含量选择了一组9个位点。在群体样本之间和群体内部均获得了高水平的多态性，中国和菲律宾的菌株似乎遵循不同的谱系，且各省之间存在明显的分支。此外，在中国大陆，基因型聚类似乎与栖息地类型和/或中间宿主形态有关。这些结果突出了微卫星在日本血吸虫群体遗传学研究中的适用性，并表明中国大陆可能存在不同株的日本血吸虫在传播。
