Williams L A, McLellan A D, Summers K L, Sorg R V, Fearnley D B, Hart D N
Haemetology Immunology Research Group, Christchurch School of Medicine, New Zealand.
Immunology. 1996 Sep;89(1):120-5. doi: 10.1046/j.1365-2567.1996.d01-720.x.
Dendritic cells (DC) are characterized as leucocytes that lack mature lineage specific markers and stimulate naive T-lymphocyte proliferation in vitro and in vivo. The mouse heat stable antigen (HSA) participates in T lymphocyte co-stimulation and is expressed by DC isolated from thymus, skin and spleen. The human HSA homologue, CD24, is predominantly expressed by B lymphocytes and granulocytes, but its expression on DC has not been studied in detail. CD24 clearly participates in B-lymphocyte signalling but co-stimulatory activity for T lymphocytes has not yet been described. We have examined the expression of CD24 on human peripheral blood DC populations isolated directly or following in vitro culture. The CD24 antigen was absent from blood DC however, cross-reactive sialylated carbohydrate epitopes were detected on DC with some CD24 monoclonal antibodies (mAb). These CD24 mAb define a protein surface antigen, which is expressed by an immature or resting subpopulation of peripheral blood DC and is down-regulated following activation differentiation in vitro.
树突状细胞（DC）的特征是缺乏成熟谱系特异性标志物的白细胞，可在体内外刺激幼稚T淋巴细胞增殖。小鼠热稳定抗原（HSA）参与T淋巴细胞共刺激，由从胸腺、皮肤和脾脏分离的DC表达。人类HSA同源物CD24主要由B淋巴细胞和粒细胞表达，但尚未详细研究其在DC上的表达。CD24明显参与B淋巴细胞信号传导，但尚未描述其对T淋巴细胞的共刺激活性。我们检测了直接分离或体外培养后人外周血DC群体上CD24的表达。血液DC中不存在CD24抗原，然而，用一些CD24单克隆抗体（mAb）在DC上检测到交叉反应性唾液酸化碳水化合物表位。这些CD24 mAb定义了一种蛋白质表面抗原，其由外周血DC的未成熟或静息亚群表达，并在体外激活分化后下调。
