Chandrashekar R, Ogunrinade A F, Weil G J
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Trop Med Int Health. 1996 Oct;1(5):575-80. doi: 10.1111/j.1365-3156.1996.tb00082.x.
The diagnostic value of ELISAs based on recombinant Onchocerca volvulus antigens OC 3.6 and OC 9.3 was evaluated with sera from endemic areas in West Africa, Guatemala and Ecuador. IgG assays were slightly more sensitive than those that detected IgG4, and the antigen combination was significantly more sensitive than either antigen alone (OC 3.6, 93%; OC 9.3, 84%, combined 98%). These assays were also evaluated with sera from 2 villages in the Onchocerciasis Control Programme area of West Africa including one village (Pendie) with recent recrudescence of infection and one (Niarba) where transmission had been interrupted for 15 years by vector control. The OC 3.6 IgG antibody assay was sensitive for new infections and exposure in Pendie; 24/24 (100%) of people with positive skin snips and 15/74 (20%) of sera from MF negative people had IgG antibodies to this antigen. In addition, antibodies to OC 3.6 often preceded the onset of skin snip positivity in Pendie. In contrast, IgG antibodies to OC 3.6 and OC 9.3 were rarely seen in children born during the 15 years since transmission was interrupted by vector control in Niarba. These encouraging results suggest that antibody assays based on OC 3.6 and OC 9.3 may be valuable tools for surveillance of onchocerciasis and also for monitoring the efficacy of control programmes.
采用来自西非、危地马拉和厄瓜多尔流行地区的血清，评估了基于重组盘尾丝虫抗原OC 3.6和OC 9.3的酶联免疫吸附测定（ELISA）的诊断价值。IgG检测比检测IgG4的检测略敏感，并且抗原组合比单独任何一种抗原都显著更敏感（OC 3.6，93%；OC 9.3，84%，联合检测为98%）。还采用来自西非盘尾丝虫病控制项目地区2个村庄的血清对这些检测进行了评估，其中一个村庄（彭迪）近期感染复发，另一个村庄（尼亚尔巴）通过病媒控制已中断传播15年。OC 3.6 IgG抗体检测对彭迪的新感染和暴露敏感；24/24（100%）皮肤切片阳性者和15/74（20%）微丝蚴阴性者的血清具有针对该抗原的IgG抗体。此外，在彭迪，针对OC 3.6的抗体通常先于皮肤切片阳性出现。相比之下，自尼亚尔巴通过病媒控制中断传播后的15年里出生的儿童中，很少见到针对OC 3.6和OC 9.3的IgG抗体。这些令人鼓舞的结果表明，基于OC 3.6和OC 9.3的抗体检测可能是盘尾丝虫病监测以及控制项目效果监测的有价值工具。
