Faure E, Lecine P, Imbert J, Champion S
Institut de Chimie Biologique, Université de Provence, Marscelle, France.
AIDS Res Hum Retroviruses. 1996 Nov 1;12(16):1519-27. doi: 10.1089/aid.1996.12.1519.
Transcription of human immunodeficiency virus type 1 (HIV-1) is regulated by multiple cis-acting regulatory elements located in the viral long terminal repeats (LTRs). HIV-1 LTR enhancer is activated by a variety of heterologous viral, chemical, and physical agents. Studies have demonstrated that irradiation by X-rays induces transcription under the control of the HIV-1 LTR and that ionizing radiations activate DNA binding of the nuclear transcription factor NF-kappa B. Using various constructs expressing a reporter gene under the control of complete or deleted LTRs of HIV-1, we evidenced that a sequence located in the U3 region was involved in X-ray activation of the HIV-1 LTR in the human colonic carcinoma cell line HT29. The cis-acting element conferring X-ray responsiveness is indistinguishable from HIV NF-kappa B tandem repeat binding sites (HIV-1, kappa B). The present work has examined the effects of X-irradiation on the NF-kappa B transcription factor. Furthermore, we characterized the subunit composition of the two major nuclear NF-kappa B complexes that bind HIV-1 kappa B after X-ray irradiation.
1型人类免疫缺陷病毒（HIV-1）的转录受位于病毒长末端重复序列（LTRs）中的多个顺式作用调节元件调控。HIV-1 LTR增强子可被多种异源病毒、化学和物理因子激活。研究表明，X射线照射可在HIV-1 LTR的控制下诱导转录，且电离辐射可激活核转录因子NF-κB的DNA结合活性。利用在HIV-1完整或缺失LTR控制下表达报告基因的各种构建体，我们证明位于U3区域的一个序列参与了人结肠癌细胞系HT29中HIV-1 LTR的X射线激活。赋予X射线反应性的顺式作用元件与HIV NF-κB串联重复结合位点（HIV-1 κB）无法区分。本研究检测了X射线照射对NF-κB转录因子的影响。此外，我们还对X射线照射后结合HIV-1 κB的两种主要核NF-κB复合物的亚基组成进行了表征。
