Gilles A, Miquelis A, Luis J, Faure E
Université de Provence, Marseille, France.
Ital J Biochem. 1998 Jun;47(2):101-10.
The ability of the human immunodeficiency virus type 1 (HIV-1) to persist and replicate in human CD4+ cells is under the control of both virally encoded proteins and a variety of host-related factors. Transcription of human immunodeficienty virus type 1 (HIV-1) is regulated by multiple cis-acting regulatory elements located in the viral long terminal repeats (LTRs). Here we report that the human vasoactive intestinal peptide (VIP) can activate the HIV-1-LTR. The activity of VIP is dose-dependent and specific. The effective concentration of VIP is within the physiological range suggesting that VIP release may modulate the activity of HIV-1 in vivo. A sequence in U3 region could be involved in VIP and in various VIP-related peptides' activation of HIV-1-LTR. This cis-acting element conferring VIP responsiveness is indistinguishable from HIV NF-kappa B tandem repeat binding sites (HIV-1 kappa B).
1型人类免疫缺陷病毒（HIV-1）在人类CD4+细胞中持续存在和复制的能力受病毒编码蛋白和多种宿主相关因素的控制。1型人类免疫缺陷病毒（HIV-1）的转录受位于病毒长末端重复序列（LTRs）中的多个顺式作用调节元件调控。在此我们报告，人类血管活性肠肽（VIP）可激活HIV-1-LTR。VIP的活性呈剂量依赖性且具有特异性。VIP的有效浓度在生理范围内，提示VIP释放可能在体内调节HIV-1的活性。U3区域中的一个序列可能参与VIP及各种VIP相关肽对HIV-1-LTR的激活。这种赋予VIP反应性的顺式作用元件与HIV NF-κB串联重复结合位点（HIV-1 κB）无法区分。
