Kagawa T, Oba A, Okumura S, Ikenaka K
National Institute for Physiological Sciences, Okazaki National Research Institutes, Aichi, Japan.
Dev Neurosci. 1996;18(4):309-18. doi: 10.1159/000111421.
UDP-galactose:ceramide galactosyltransferase (CGT) is considered to be a key enzyme in the biosynthesis of galactocerebroside (GalC), a frequently used marker for oligodendrocytes. CGT gene expression has been shown to be restricted to the oligodendrocyte lineage in young adult rat brain. We detected expression of the CGT gene in embryonic mouse brain and spinal cord from E17 onward using an in situ hybridization technique. Localization of CGT messages was restricted to two longitudinal columns of cells in the basal half of the ventricular zone around the fourth ventricule at the most caudal level of the medulla oblongata. After birth, they were distributed in caudal-to-rostral and ventral-to-dorsal gradients. This study shows that CGT mRNA is a good marker for localizing oligodendrocytes, and appears just before the GalC-positive stage.
UDP-半乳糖：神经酰胺半乳糖基转移酶（CGT）被认为是半乳糖脑苷脂（GalC）生物合成中的关键酶，GalC是少突胶质细胞常用的标志物。已证明CGT基因表达在成年幼鼠大脑中局限于少突胶质细胞谱系。我们使用原位杂交技术检测了从胚胎第17天起胚胎小鼠脑和脊髓中CGT基因的表达。CGT信息的定位局限于延髓最尾端水平第四脑室周围室管膜区下半部的两列纵向细胞。出生后，它们呈尾端到头端和腹侧到背侧的梯度分布。本研究表明，CGT mRNA是定位少突胶质细胞的良好标志物，且在GalC阳性阶段之前出现。
