Shin D H, Kim S, Lee W J, Park K H, Jeon G S, Lee K H, Baik S H, Cho S S
Department of Anatomy, Seoul National University College of Medicine, Korea.
Anat Embryol (Berl). 1999 Aug;200(2):193-201. doi: 10.1007/s004290050272.
We studied the expression of UDP-galactose: ceramide galactosyl transferase (CGT) mRNA in postnatal rat brains using an in situ hybridization technique. From P0 to P16, there was a defined temporal and spatial pattern to the earliest acquisition of CGT mRNA expression. In the forebrain, CGT mRNA-expressing (CGT+) cells were first detected in regions outside the subventricular zone around the lateral ventricle at P2. Cells in the external capsule, internal capsule and corpus callosum were later found to be CGT-positive. At P8 to P16, CGT+ cells were found in the thalamus, striatum, occipital and frontal cortex. In the case of midbrain and hindbrain, the first CGT+ signals were detected in the medullary raphe of the medulla oblongata at P0. CGT+ cells were subsequently located in the cerebellum, midbrain and pons from P4 to P16. That is, in regions closer to the areas in which CGT+ cells were first found, CGT mRNA expression was observed much earlier. These findings support the notion that there are at least two discrete waves of CGT mRNA signal expression in the forebrain and hindbrain.
我们采用原位杂交技术研究了出生后大鼠脑内UDP-半乳糖：神经酰胺半乳糖基转移酶（CGT）mRNA的表达情况。从出生后第0天（P0）到第16天（P16），CGT mRNA表达最早的获得呈现出特定的时间和空间模式。在前脑，P2时首次在侧脑室室管膜下区以外的区域检测到表达CGT mRNA的（CGT+）细胞。随后发现外囊、内囊和胼胝体中的细胞为CGT阳性。在P8至P16时，在丘脑、纹状体、枕叶和额叶皮质中发现了CGT+细胞。在中脑和后脑，P0时在延髓的髓质中缝首次检测到CGT+信号。从P4到P16，CGT+细胞随后定位于小脑、中脑和脑桥。也就是说，在更接近首次发现CGT+细胞的区域，CGT mRNA表达更早被观察到。这些发现支持了在前脑和后脑至少有两波离散的CGT mRNA信号表达的观点。
