de Souza J M, DeVoe R D, Schoeps C, Ventura D F
Department of Experimental Psychology, University of São Peulo, Brazil.
J Neurosci Methods. 1996 Oct;68(2):203-10. doi: 10.1016/0165-0270(96)00076-3.
A number of methods have been used in the past to measure spectral sensitivity (S(lambda)) functions of electric responses in the visual system. We present here a microcomputer based, AC, constant-response method for automatic on-line measurement of S(lambda) in cells with or without a sustained tonic response. It is based on feedback adjustment of light intensity to obtain constant peak-to-peak amplitudes of response to a flickering stimulus as the spectrum is scanned between 300 and 700 nm in 4 nm steps. It combines the advantages of: (1) on-line presentation of S(lambda) curves; (2) constant light adaptation; (3) sampling of many points; and (4) fast data collection time. The system can be applied to sensitivity or threshold (e.g., S(lambda), dark adaptation, receptive field) measurements of any electrically recorded visual response.
