Subclinical versus clinical hepatitis in the dobermann: evaluation of changes in blood parameters.

Concentrations of the enzymes alanine aminotransferase (ALT) and alkaline phosphatase (AP) were determined in blood samples from 626 randomly selected clinically healthy dobermann. ALT levels greater than three times the normal upper value were detected in 55 dogs. These dogs were selected for further investigation; the owners of 23 of the dogs allowed a liver biopsy to be performed. Histopathological examination revealed various degrees of hepatitis and excessive amounts of copper in 21 of the dogs. These cases, referred to as subclinical dobermann hepatitis (DH), were selected for a follow-up investigation in which the clinical signs and serum parameters (ALT, AP and bilirubin) were studied for a period of three to 48 months. Serum parameters of those with subclinical DH were compared with blood samples collected from 22 dogs with clinical DH. Individual dogs showed great variation in the levels of ALT and AP between consecutive serum samples. These enzyme levels never, however, fell to the normal range. During the subclinical stage no statistically significant (P > 0.05) change occurred in the concentrations of ALT or AP. When dogs with subclinical DH were compared with dogs with clinical DH, there was no statistically significant (P > 0.05) difference in ALT levels, whereas AP concentrations were significantly (P < 0.001) higher among clinically affected dogs. Elevated levels of bilirubin were detected almost exclusively in dogs with clinical DH. After the onset of clinical signs there was a decrease in the ALT levels and an increase in AP concentrations as the disease progressed, but the changes were not statistically significant (P > 0.05).