Department of Veterinary Parasitology and Entomology, University of Nigeria, Nsukka, Nigeria.
Vet Parasitol. 2012 Nov 23;190(1-2):56-61. doi: 10.1016/j.vetpar.2012.05.012. Epub 2012 May 23.
The serum activities of alkaline phosphatase (AP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and the serum levels of conjugated bilirubin (CB), blood urea nitrogen (BUN) and creatinine were studied following single and mixed infections of mongrel dogs with Trypanosoma congolense and Trypanosoma brucei brucei. Twenty mongrel dogs of both sexes aged between 3 and 6 months, and weighing between 2.5 and 5.9 kg were used for the study. The dogs were kept in clean metal cages in a fly-proof house and were fed and given water ad libitum. The twenty dogs were divided into four groups of five dogs each. Group I dogs were uninfected control, group II were infected with T. congolense, group III were infected with T. brucei brucei and group IV were infected with both T. congolense and T. brucei brucei. Each dog in the infected groups II and III was inoculated intraperitonealy (i/p) with 1.0 ml of PBS diluted blood containing 1.0×10(6) trypanosomes whereas each infected dog in group IV (mixed infection) was inoculated with 0.5 ml of the PBS diluted blood containing 0.5×10(6)T. congolense and 0.5 ml of the PBS diluted blood containing 0.5×10(6)T. brucei brucei i/p. Parasites were detectable in the blood of the infected dogs in groups II, III, and IV 10-13 days post infection (PI) with the mean pre-patent period (PP) of 12, 10, and 11 days respectively. Trypanosome infection caused a significant (P<0.05) increase in the serum activities of AP, ALT, AST and the serum levels of creatinine, CB, and BUN. The significant increases in the serum levels of CB, BUN, and creatinine and serum activities of AP and AST became noticeable from day seven PI in all the infected groups whereas that of ALT became noticeable from day 14 PI and increased continuously until the experiment was terminated. These increases however did not differ significantly (P>0.05) between the infected groups in most cases. It was thus concluded that single or mixed infection of mongrel dogs with T. congolense and T. brucei brucei resulted in significant increases in the serum activities of AP, AST, ALT and serum levels of creatinine, CB and BUN which in most cases did not differ significantly (P>0.05) among the infected groups.
本研究旨在探讨混合感染和单一感染刚地弓形虫（Trypanosoma congolense）和布氏冈比亚锥虫（Trypanosoma brucei brucei）的杂种犬的血清碱性磷酸酶（AP）、丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）活性和结合胆红素（CB）、血尿素氮（BUN）和肌酐水平的变化。选用 20 只 3-6 月龄、体重 2.5-5.9kg 的杂种犬，雌雄不拘，饲养于带纱窗的清洁金属笼中，自由采食和饮水。将 20 只犬随机分为 4 组，每组 5 只。第 I 组为未感染对照组，第 II 组感染 T. congolense，第 III 组感染 T. brucei brucei，第 IV 组混合感染 T. congolense 和 T. brucei brucei。第 II 组和第 III 组感染犬经腹腔（i/p）接种含 1.0×10(6)个锥虫的 1.0ml PBS 稀释血，第 IV 组（混合感染组）则分别接种含 0.5ml 1.0×10(6)个 T. congolense 和 0.5ml 1.0×10(6)个 T. brucei brucei 的 PBS 稀释血。感染后第 10-13 天，第 II、III 和 IV 组感染犬血液中均可检测到寄生虫，潜伏前期（PP）分别为 12、10 和 11 天。锥虫感染导致血清 AP、ALT、AST 活性和肌酐、CB、BUN 水平显著升高（P<0.05）。所有感染组犬血清 CB、BUN 和肌酐水平及 AP 和 AST 活性于感染后第 7 天开始显著升高，而 ALT 则于第 14 天开始显著升高，并持续升高至实验结束。大多数情况下，各组间差异无统计学意义（P>0.05）。因此，混合或单一感染杂种犬可导致血清 AP、AST、ALT 活性和肌酐、CB、BUN 水平显著升高，大多数情况下，各组间差异无统计学意义（P>0.05）。
