Gillery P, Georges N, Randoux A, Lefèvre F, Maquart F X, Borel J P
Laboratory of Biochemistry and Molecular Biology, CNRS EP 89, IFR 53-Biomolecules, Faculty of Medicine, University of Reims Champagne-Ardenne, France.
Biochem Biophys Res Commun. 1996 Nov 1;228(1):94-9. doi: 10.1006/bbrc.1996.1621.
Fibroblasts cultivated in a collagen matrix exhibit a large decrease in the synthesis of most proteins, depending on transcriptional and posttranscriptional controls. We have previously shown that ribosomal RNA content and half-life were decreased in collagen lattice cultures. Here, we cultivated human dermal fibroblasts in monolayers and in lattices and studied by competitive RT-PCR analysis the expression of the nucleolar proteins nucleolin and fibrillarin, two key factors in ribosome processing and association. Nucleolin expression was found increased, and fibrillarin expression decreased, in collagen-lattice vs monolayer-cultured fibroblasts, with some variability according to the strains (+25 to +250% and -40 to -60%, respectively). These data suggest that a possible trouble of the association between neosynthesized rRNA and nucleolar proteins is, at least partly, responsible for the inhibition of protein synthesis induced by the extracellular matrix.
在胶原基质中培养的成纤维细胞，其大多数蛋白质的合成大幅减少，这取决于转录和转录后调控。我们之前已经表明，在胶原晶格培养物中核糖体RNA含量和半衰期会降低。在这里，我们将人皮肤成纤维细胞培养成单层和晶格形式，并通过竞争性逆转录-聚合酶链反应分析研究核仁蛋白核仁素和纤维原蛋白的表达，这两种蛋白是核糖体加工和组装的关键因子。与单层培养的成纤维细胞相比，在胶原晶格培养的成纤维细胞中发现核仁素表达增加，纤维原蛋白表达减少，不同菌株存在一定差异（分别为+25%至+250%和-40%至-60%）。这些数据表明，新合成的rRNA与核仁蛋白之间关联的可能异常至少部分导致了细胞外基质诱导的蛋白质合成抑制。
