人类颗粒蛋白/上皮生成素基因启动子的结构与功能分析
Structural and functional analysis of a promoter of the human granulin/epithelin gene.
作者信息
Bhandari V, Daniel R, Lim P S, Bateman A
机构信息
Endocrine Laboratory, Royal Victoria Hospital, Montreal, Quebec, Canada.
出版信息
Biochem J. 1996 Oct 15;319 ( Pt 2)(Pt 2):441-7. doi: 10.1042/bj3190441.
Abstract
Granulins (grns) or epithelins (epis) are peptides with molecular masses of approx. 6 kDa that modulate the growth of cells. The precursor for the grns/epis, which might itself be biologically active, is a secreted glycoprotein containing multiple repeats of the grn/epi motif. Grn/epi mRNA occurs widely in vivo, particularly in tissues rich in epithelial and haematopoietic cells. To understand better the role of the gene products for grn/epi it is important to determine the patterns of grn/epi gene expression and how this is regulated. To assist in this we have obtained the 5' sequence of the human grn/epi gene, and using chimaeras of the grn/epi -5' sequence and the chloramphenicol acetyltransferase gene we have shown a strong promoter activity associated with the 5' sequence of the human grn/epi gene. We have further delineated regions of the 5' sequence that confer high-level expression on the chimaeric gene.
摘要
颗粒蛋白（grns）或上皮素（epis）是分子量约为6 kDa的肽，可调节细胞生长。grns/epis的前体本身可能具有生物活性，是一种分泌型糖蛋白，含有多个grn/epi基序重复序列。Grn/epi mRNA在体内广泛存在，尤其是在上皮细胞和造血细胞丰富的组织中。为了更好地理解grn/epi基因产物的作用，确定grn/epi基因表达模式及其调控方式非常重要。为了辅助这一研究，我们获得了人类grn/epi基因的5'序列，并利用grn/epi -5'序列与氯霉素乙酰转移酶基因的嵌合体，证明了与人类grn/epi基因5'序列相关的强启动子活性。我们进一步划定了5'序列中赋予嵌合基因高水平表达的区域。
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