核小体核心颗粒抑制DNA三螺旋的形成。
Nucleosome core particles inhibit DNA triple helix formation.
作者信息
Brown P M, Fox K R
机构信息
Department of Physiology and Pharmacology, University of Southampton, U.K.
出版信息
Biochem J. 1996 Oct 15;319 ( Pt 2)(Pt 2):607-11. doi: 10.1042/bj3190607.
Abstract
We have used DNase I footprinting to examine the formation of DNA triple helices at target sites on DNA fragments that have been reconstituted with nucleosome core particles. We show that a 12 bp homopurine target site, located 45 bp from the end of the 160 bp tyrT(46A) fragment, cannot be targeted with either parallel (CT-containing) or antiparallel (GT-containing) triplex-forming oligonucleotides when reconstituted on to nucleosome core particles. Binding is not facilitated by the presence of a triplex-binding ligand. However, both parallel and antiparallel triplexes could be formed on a truncated DNA fragment in which the target site was located closer to the end of the DNA fragment. We suggest that intermolecular DNA triplexes can only be formed on those DNA regions that are less tightly associated with the protein core.
摘要
我们利用DNA酶I足迹法来检测在已用核小体核心颗粒重构的DNA片段上的靶位点处DNA三链螺旋的形成。我们发现，位于160 bp tyrT(46A)片段末端45 bp处的一个12 bp同型嘌呤靶位点，在重构到核小体核心颗粒上时，不能被平行（含CT）或反平行（含GT）三链形成寡核苷酸靶向。三链结合配体的存在并不能促进结合。然而，平行和反平行三链体都可以在一个截短的DNA片段上形成，其中靶位点更靠近DNA片段的末端。我们认为分子间DNA三链体只能在那些与蛋白质核心结合不太紧密的DNA区域形成。
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