Brown P M, Fox K R
Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, U.K.
Biochem J. 1998 Jul 15;333 ( Pt 2)(Pt 2):259-67. doi: 10.1042/bj3330259.
We have used DNase I and hydroxyl-radical footprinting to examine the formation of intermolecular DNA triple helices on nucleosome-bound DNA fragments containing An.Tn tracts. We found that it is possible to form triplexes on these nucleosome-bound DNAs, but the stability of the complexes depends on the orientation of the A tract with respect to the protein surface. Hydroxyl-radical cleavage of these complexes suggests that the DNA fragments are still associated with the nucleosome. However, the phased cleavage pattern is lost in the vicinity of the triplex, suggesting that the DNA has locally moved away from the protein surface.
我们使用脱氧核糖核酸酶I和羟基自由基足迹法来检测在含有An.Tn序列的核小体结合DNA片段上分子间DNA三链螺旋的形成。我们发现有可能在这些核小体结合的DNA上形成三链体，但复合物的稳定性取决于A序列相对于蛋白质表面的方向。这些复合物的羟基自由基切割表明DNA片段仍与核小体相关联。然而，在三链体附近，相位切割模式消失，这表明DNA已局部地从蛋白质表面移开。
