Redlich C A, Grauer J N, Van Bennekum A M, Clever S L, Ponn R B, Blaner W S
Yale University School of Medicine, New Haven, Connecticut, USA.
Am J Respir Crit Care Med. 1996 Nov;154(5):1436-43. doi: 10.1164/ajrccm.154.5.8912761.
Increasing evidence suggests that beta-carotene, retinol (vitamin A), and alpha-tocopheral (vitamin E) may have important protective effects in the lung. However, surprisingly little is known about their storage and metabolism in human lung. Levels of beta-carotene, retinol, and alpha-tocopherol in human lung tissues and bronchoalveolar lavage (BAL) cells were determined with reverse-phase high-pressure liquid chromatography (HPLC). Fresh lung tissue, serum, and dietary questionnaires were obtained from 21 patients undergoing open lung surgery, and BAL cells from 12 of these patients. Dietary and serum levels of carotenoids, beta-carotene, retinol, and alpha-tocopherol were consistent with previously reported values. Lung tissue levels of total carotenoids, beta-carotene, retinol, and alpha-tocopherol were respectively 0.34 +/- 0.36 microg/g, 0.13 +/- 0.27 microg/g, 0.15 +/- 0.06 microg/g, and 9.60 +/- 4.86 microg/g tissue. Levels of these nutrients were also measured in BAL cells to establish potential markers for their lung tissue levels. Correlations between serum, BAL-cell, tissue, and dietary levels of the nutrients were determined. Lung tissue levels of total carotenoids, beta-carotene, and alpha-tocopherol, but not retinol, correlated well with their serum levels. Lung tissue levels of retinol and alpha-tocopherol correlated with their BAL-cell levels. These studies demonstrate quantifiable levels of retinol, alpha-tocopherol, and total carotenoids or beta-carotene in human lung tissue and BAL cells, and show that serum and/or BAL-cell levels of these nutrients can potentially be used to predict their lung tissue levels.
越来越多的证据表明，β-胡萝卜素、视黄醇（维生素A）和α-生育酚（维生素E）可能对肺部具有重要的保护作用。然而，令人惊讶的是，人们对它们在人肺中的储存和代谢了解甚少。采用反相高效液相色谱法（HPLC）测定人肺组织和支气管肺泡灌洗（BAL）细胞中β-胡萝卜素、视黄醇和α-生育酚的水平。从21例接受开胸肺手术的患者获取新鲜肺组织、血清和饮食调查问卷，并从其中12例患者获取BAL细胞。类胡萝卜素、β-胡萝卜素、视黄醇和α-生育酚的饮食和血清水平与先前报道的值一致。肺组织中总类胡萝卜素、β-胡萝卜素、视黄醇和α-生育酚的水平分别为0.34±0.36μg/g、0.13±0.27μg/g、0.15±0.06μg/g和9.60±4.86μg/g组织。还在BAL细胞中测量了这些营养素的水平，以确定其肺组织水平的潜在标志物。测定了血清、BAL细胞、组织和饮食中这些营养素水平之间的相关性。肺组织中总类胡萝卜素、β-胡萝卜素和α-生育酚（而非视黄醇）的水平与其血清水平密切相关。肺组织中视黄醇和α-生育酚的水平与其BAL细胞水平相关。这些研究证明了人肺组织和BAL细胞中视黄醇、α-生育酚以及总类胡萝卜素或β-胡萝卜素的可量化水平，并表明这些营养素的血清和/或BAL细胞水平可潜在地用于预测其肺组织水平。
