Ausiello C M, la Sala A, Ramoni C, Urbani F, Funaro A, Malavasi F
Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanita', Rome, Italy.
Cell Immunol. 1996 Nov 1;173(2):192-7. doi: 10.1006/cimm.1996.0267.
Human CD38, a surface glycoprotein expressed by different immunocompetent cells, is associated with distinct transmembrane signaling molecules and plays a key role in the synthesis of cyclic ADP-ribose, a calcium-mobilizing compound. This study reports that CD38 ligation by specific monoclonal antibodies (mAb) in purified peripheral blood T cells is followed by secretion of discrete cytokines. IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), IFN-gamma, and IL-10 mRNA expression were constant findings. Low levels of IL-2 mRNA were also detected in CD38-activated T lymphocyte cultures of all subjects studied. Low levels of IL-4 and IL-5 mRNA were detected in the majority of CD38-activated T cultures. Moreover, CD38 mediated cytokine induction does not require T cell proliferation or the addition of antigen presenting cells. In conclusion, human CD38 runs an activation pathway in purified T cells which operates through the induction of a cytokine profile shared by Th1 or Th2 cells.
人CD38是一种由不同免疫活性细胞表达的表面糖蛋白，与不同的跨膜信号分子相关，并在环状ADP核糖（一种钙动员化合物）的合成中起关键作用。本研究报告称，在纯化的外周血T细胞中，特异性单克隆抗体（mAb）与CD38结合后会分泌离散的细胞因子。白细胞介素-6（IL-6）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、干扰素-γ（IFN-γ）和白细胞介素-10（IL-10）的信使核糖核酸（mRNA）表达是持续观察到的结果。在所有研究对象的CD38激活的T淋巴细胞培养物中也检测到低水平的白细胞介素-2（IL-2）mRNA。在大多数CD38激活的T细胞培养物中检测到低水平的白细胞介素-4（IL-4）和白细胞介素-5（IL-5）mRNA。此外，CD38介导的细胞因子诱导不需要T细胞增殖或添加抗原呈递细胞。总之，人CD38在纯化的T细胞中运行一条激活途径，该途径通过诱导Th1或Th2细胞共有的细胞因子谱来发挥作用。
