Hiroi T, Watabe S, Takimoto K, Yago N, Yamamoto Y, Takahashi S Y
Radioisotope Research Institute, St. Marianna University School of Medicine, Kawasaki, Japan.
DNA Seq. 1996;6(4):239-42. doi: 10.3109/10425179609008449.
We have isolated cDNA clones coding SP-22, an antioxidant protein in mitochondria, from a bovine adrenal medulla cDNA library constructed with (lambda)gt11. The largest clone contained the entire coding sequence for mature SP-22. Since the isolated cDNA clones lacked 5'- and 3'-ends, we determined the sequences of both ends by the "Rapid Amplification of cDNA Ends (RACE)" tecnique. The deduced amino acid sequence of the mature protein region was the same as that determined by protein sequencing. Since SP-22 had a mitochondrial targetting signal, its mitochondrial localization was confirmed.
我们从用λgt11构建的牛肾上腺髓质cDNA文库中分离出了编码线粒体抗氧化蛋白SP - 22的cDNA克隆。最大的克隆包含成熟SP - 22的完整编码序列。由于分离出的cDNA克隆缺少5'端和3'端，我们通过“cDNA末端快速扩增（RACE）”技术确定了两端的序列。成熟蛋白区域推导的氨基酸序列与蛋白质测序确定的序列相同。由于SP - 22具有线粒体靶向信号，其线粒体定位得到了证实。
