Morris J C, Neben S, Bennett F, Finnerty H, Long A, Beier D R, Kovacic S, McCoy J M, DiBlasio-Smith E, La Vallie E R, Caruso A, Calvetti J, Morris G, Weich N, Paul S R, Crosier P S, Turner K J, Wood C R
Genetics Institute, Inc., Cambridge, MA 02140, USA.
Exp Hematol. 1996 Oct;24(12):1369-76.
Human interleukin-11 (IL-11) has been shown to have pleiotropic action on hematopoietic, hepatic, stromal, epithelial, neural, and osteoclast cells. In the present work, the murine IL-11 cDNA has been isolated from a fetal thymic cell line, and its structure and function compared with human IL-11. The murine protein was demonstrated to have identical actions on the proliferation of a murine plasmacytoma cell line, murine primitive bone marrow progenitor cells, and megakaryocyte precursors. The murine IL-11 protein was synthesized as a soluble thioredoxin-IL-11 fusion in Escherichia coli and the expression of murine IL-11 was examined by pulse-chase radiolabeling in COS cells. The chromosomal location of the murine IL-11 gene was assigned to the proximal arm of chromosome 7.
人白细胞介素-11（IL-11）已被证明对造血细胞、肝细胞、基质细胞、上皮细胞、神经细胞和破骨细胞具有多效性作用。在本研究中，从小鼠胎儿胸腺细胞系中分离出小鼠IL-11 cDNA，并将其结构和功能与人类IL-11进行比较。已证明该小鼠蛋白对小鼠浆细胞瘤细胞系、小鼠原始骨髓祖细胞和巨核细胞前体的增殖具有相同的作用。小鼠IL-11蛋白在大肠杆菌中合成为可溶性硫氧还蛋白-IL-11融合蛋白，并通过在COS细胞中进行脉冲追踪放射性标记来检测小鼠IL-11的表达。小鼠IL-11基因的染色体定位被确定在7号染色体的近端臂上。
