两种牛科动物的核仁组织区

Nucleolus organizer regions in two species of Bovidae.

作者信息

Di Berardino D, Arrighi F E, Kieffer N M

出版信息

J Hered. 1979 Jan-Feb;70(1):47-50. doi: 10.1093/oxfordjournals.jhered.a109187.

DOI:10.1093/oxfordjournals.jhered.a109187

Abstract

Silver-staining of nucleolar organizer regions (NORs) in somatic cells of Holstein cattle (Bos taurus) and European buffalo Murrah type (Bubalus bubalus) indicates that the NORs are located in the telomeres of chromosomes 2,3,4,11, and 29 of cattle and chromosomes 3,4,6,23, and 24 of buffalo. Chromosome identification was by Q-banding. Analysis of 326 metaphase cells showed a mean value of 7.7 +/- 1.2 for Ag-NORs with a range between 4 and 10 for cattle. In all of the cells examined, either one or both homologues of chromosome 29 showed telomeric silver staining for cattle. Meiotic preparations showed not more than 5 clusters of silver grains in the pachytene stage of cattle.

摘要

对荷斯坦奶牛（Bos taurus）和欧洲穆拉水牛（Bubalus bubalus）体细胞中的核仁组织区（NORs）进行银染，结果表明，NORs位于奶牛2号、3号、4号、11号和29号染色体的端粒以及水牛3号、4号、6号、23号和24号染色体的端粒上。通过Q带进行染色体鉴定。对326个中期细胞的分析显示，奶牛的Ag-NORs平均值为7.7±1.2，范围在4到10之间。在所有检测的细胞中，奶牛29号染色体的一条或两条同源染色体均显示端粒银染。减数分裂制片显示，奶牛粗线期的银粒簇不超过5个。