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通过连续R显带和荧光原位杂交对牛和水牛中ω干扰素基因及滋养层干扰素基因进行染色体定位。

Chromosomal localization of omega and trophoblast interferon genes in cattle and river buffalo by sequential R-banding and fluorescent in situ hybridization.

作者信息

Iannuzzi L, Gallagher D S, Ryan A M, Di Meo G P, Womack J E

机构信息

Consiglio Nazionale delle Ricerche, Istituto di Adattamento dei Bovini e dei Bufali all'Ambiente del Mezzogiorno (CNR-IABBAM), Naples, Italy.

出版信息

Cytogenet Cell Genet. 1993;62(4):224-7. doi: 10.1159/000133482.

DOI:10.1159/000133482
PMID:8440140
Abstract

Fluorescent in situ hybridization (FISH) of the cattle cDNA probe bTP-509 to RBA-banded cattle (Bos taurus L.) chromosomes confirmed the assignment of the omega (IFNW) and trophoblast (IFNT) interferon genes to chromosome 8q15. Using the same probe, these genes were also localized to river buffalo (Bubalus bubalis L.) chromosome 3q15 following sequential RBA-banding and FISH. The extensive G- and R-banding homology observed between cattle chromosome 8 and river buffalo chromosome arm 3q supports the conserved chromosomal location of the IFNW and IFNT genes in these two species.

摘要

将牛的cDNA探针bTP - 509与经RBA显带的牛(Bos taurus L.)染色体进行荧光原位杂交(FISH),证实了ω(IFNW)和滋养层(IFNT)干扰素基因定位于8号染色体q15。使用相同的探针,在连续进行RBA显带和FISH后,这些基因也定位于河水牛(Bubalus bubalis L.)的3号染色体q15。在牛8号染色体和河水牛3号染色体臂3q之间观察到广泛的G带和R带同源性,支持了这两个物种中IFNW和IFNT基因在染色体上的保守定位。

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