Pestaner J P, Mullick F G, Centeno J A
Office of Chief Medical Examiner of the City of New York, New York, USA.
J Forensic Sci. 1996 Nov;41(6):1060-3.
The in situ spectroscopic identification of acetaminophen in a fatal overdose case is described. Numerous techniques have been used to analyze acetaminophen in biological fluids, however, the use of nondestructive spectroscopic techniques has not been documented. In this investigation, the demonstration of the drug material was established by using the laser Raman microprobe technique, providing an accurate identification by virtue of the drug's molecular fingerprint characteristics. Material found on the deceased was collected and placed on metal (aluminum-coated) plated slides and excited with the 514.5 nm line of an argon ion laser, which was focused to a 1 micron spot size using a high-resolution optical microscope. Spectra of acetaminophen particles with an average size of 5 to 8 microns were obtained. The Raman spectrum of this drug contains characteristic group frequencies assigned to the C = O at 1649 cm-1, the N-H deformation mode at 1620 to 1612 cm-1, the bendstretch mode of the H-N-C = O at 1562 cm-1, the C-H bending mode at 1325 cm-1, and the phenyl ring stretch at 799 cm-1, respectively. The results reported here demonstrate the capability of laser Raman microprobe as a useful adjunct tool for the identification of foreign materials in forensic pathology.
本文描述了在一例致命性过量服用对乙酰氨基酚案件中对其进行原位光谱鉴定的情况。已有多种技术用于分析生物体液中的对乙酰氨基酚，然而，无损光谱技术的应用尚无文献记载。在本研究中，利用激光拉曼微探针技术确定了药物物质的存在，凭借药物的分子指纹特征实现了准确鉴定。从死者身上采集的物质被收集起来，放置在金属（镀铝）载玻片上，并用氩离子激光的514.5 nm谱线进行激发，使用高分辨率光学显微镜将其聚焦到1微米的光斑尺寸。获得了平均尺寸为5至8微米的对乙酰氨基酚颗粒的光谱。该药物的拉曼光谱分别包含归属于1649 cm-1处的C = O、1620至1612 cm-1处的N - H变形模式、1562 cm-1处的H - N - C = O弯曲拉伸模式、1325 cm-1处的C - H弯曲模式以及799 cm-1处的苯环拉伸的特征基团频率。此处报道的结果证明了激光拉曼微探针作为法医病理学中鉴定异物的有用辅助工具的能力。
