Bryden A S, Bertrand J
Public Health Laboratory Service North West, Preston, England, UK.
Br J Biomed Sci. 1996 Sep;53(3):182-4.
The diagnosis of adenovirus conjunctivitis using a commercial enzyme immunoassay (EIA) was investigated and compared with virus isolation in cell culture. Of 53 eye swabs previously shown to be adenovirus-positive, EIA was positive with 33 and isolation in PLC/PRF/5 cells was successful with 49. Isolates were typed by serum neutralisation. Adenovirus types 3 and 4 were most common, with 14 and 12 isolates respectively. Nine isolates were found to be type 8 and eight were classified as untyped following unsuccessful neutralisation by antisera to types 1-8 and 14. The potential of EIA, and possible modifications to increase sensitivity, are discussed.
研究了使用商业酶免疫测定法（EIA）诊断腺病毒结膜炎，并与细胞培养中的病毒分离法进行了比较。在先前显示为腺病毒阳性的53份眼拭子中，EIA检测出33份呈阳性，在PLC/PRF/5细胞中成功分离出49份。通过血清中和法对分离株进行分型。3型和4型腺病毒最为常见，分别有14份和12份分离株。发现9份分离株为8型，8份在使用1 - 8型和14型抗血清进行中和试验未成功后被归类为未分型。讨论了EIA的潜力以及可能提高其灵敏度的改进方法。
