Panaud O, Chen X, McCouch S R
Department of Plant Breeding, Cornell University, Ithaca, NY 14853-1901, USA.
Mol Gen Genet. 1996 Oct 16;252(5):597-607. doi: 10.1007/BF02172406.
Microsatellite markers containing simple sequence repeats (SSR) are a valuable tool for genetic analysis. Our objective is to augment the existing RFLP map of rice with simple sequence length polymorphisms (SSLP). In this study, we describe 20 new microsatellite markers that have been assigned to positions along the rice chromosomes, characterized for their allelic diversity in cultivated and wild rice, and tested for amplification in distantly related species. Our results indicate that the genomic distribution of microsatellites in rice appears to be random, with no obvious bias for, or clustering in particular regions, that mapping results are identical in intersubspecific and interspecific populations, and that amplification in wild relatives of Oryza sativa is reliable in species most closely related to cultivated rice but becomes less successful as the genetic distance increases. Sequence analysis of SSLP alleles in three related indica varieties demonstrated the clustering of complex arrays of SSR motifs in a single 300-bp region with independent variation in each. Two microsatellite markers amplified multiple loci that were mapped onto independent rice chromosomes, suggesting the presence of duplicated regions within the rice genome. The availability of increasing numbers of mapped SSLP markers can be expected to increase the power and resolution of genome analysis in rice.
含有简单序列重复（SSR）的微卫星标记是遗传分析的宝贵工具。我们的目标是用简单序列长度多态性（SSLP）来扩充现有的水稻RFLP图谱。在本研究中，我们描述了20个新的微卫星标记，它们已被定位到水稻染色体上的位置，对其在栽培稻和野生稻中的等位基因多样性进行了表征，并测试了在远缘物种中的扩增情况。我们的结果表明，水稻中微卫星的基因组分布似乎是随机的，没有明显偏向或聚集在特定区域，种间和种内群体的定位结果相同，并且在栽培稻最密切相关的物种中，水稻野生近缘种的扩增是可靠的，但随着遗传距离的增加成功率会降低。对三个相关籼稻品种中SSLP等位基因的序列分析表明，SSR基序的复杂阵列聚集在一个300 bp的单一区域，每个区域具有独立变异。两个微卫星标记扩增出多个位点，这些位点被定位到独立的水稻染色体上，表明水稻基因组中存在重复区域。预计越来越多已定位的SSLP标记的可用性将提高水稻基因组分析的能力和分辨率。
