Hayashi S, Miharu N, Okamoto E, Samura O, Hara T, Ohama K
Department of Obstetrics and Gynecology, Hiroshima University School of Medicine, Japan.
Jpn J Hum Genet. 1996 Mar;41(1):193-202. doi: 10.1007/BF01892627.
Fifty uterine leiomyomas were examined using conventional cytogenetic method and fluorescence in situ hybridization (FISH) for detection of chromosomal abnormalities of chromosome 12. Of the 50 tumors, nine were examined using FISH on the non-cultured samples. Two (4.0%) of 50 tumor samples examined showed chromosomal abnormalities of chromosome 12 by the conventional cytogenetic analysis. For FISH, the whole-chromosome painting probe and D12Z3 probe specific for the centromeric region were used. Of the 50 cultured samples, 10 showed structural aberrations and four showed numerical aberrations of chromosome 12 by FISH analysis. Of the nine non-cultured samples, four showed structural abnormalities of chromosome 12, all of which also showed structural abnormalities of chromosome 12 on the cultured samples. These results indicate that chromosomal abnormalities of chromosome 12 are important in the biology of at least some types of uterine leiomyoma, and that FISH is a useful complement to the conventional cytogenetic analysis in the study of solid tumors.
采用传统细胞遗传学方法和荧光原位杂交（FISH）技术对50例子宫平滑肌瘤进行检测，以检测12号染色体的染色体异常情况。在这50个肿瘤样本中，有9个样本采用非培养样本进行FISH检测。通过传统细胞遗传学分析，50个肿瘤样本中有2个（4.0%）显示12号染色体存在染色体异常。对于FISH检测，使用了全染色体涂染探针和针对着丝粒区域的D12Z3探针。在50个培养样本中，通过FISH分析，有10个显示12号染色体存在结构畸变，4个显示12号染色体存在数目畸变。在9个非培养样本中，有4个显示12号染色体存在结构异常，所有这些样本在培养样本中也显示12号染色体存在结构异常。这些结果表明，12号染色体的染色体异常在至少某些类型的子宫平滑肌瘤生物学特性中具有重要意义，并且FISH在实体瘤研究中是对传统细胞遗传学分析的一种有用补充。
