用聚乙二醇修饰大肠杆菌B谷胱甘肽合成酶，用于谷胱甘肽缺乏症酶替代疗法的临床应用。
Modification of Escherichia coli B glutathione synthetase with polyethylene glycol for clinical application to enzyme replacement therapy for glutathione deficiency.
作者信息
Inoue Y, Sugiyama K, Ueminami H, Izawa S, Kimura A
机构信息
Research Institute for Food Science, Kyoto University, Japan.
出版信息
Clin Diagn Lab Immunol. 1996 Nov;3(6):663-8. doi: 10.1128/cdli.3.6.663-668.1996.
Glutathione synthetase of Escherichia coli B was modified with polyethylene glycol, and the properties of the resultant modified enzyme were investigated. The thermal stability of the modified enzyme and its resistance against several proteases increased compared with those of the native enzyme. The modified enzyme was injected intravenously via the rat tail vein, and the circulating life of the enzyme in plasma was monitored. The half-life of the native enzyme was 50 min, whereas that of the modified enzyme was approximately 24 h. The systemic anaphylaxis reaction was tested by using rats intravenously injected with the native and modified enzymes. For the native enzyme, strong reactions such as dyspnea and tumble were observed; however, no symptom or only a very weak reaction, such as scratching, was observed with the modified enzyme.
用聚乙二醇对大肠杆菌B的谷胱甘肽合成酶进行修饰，并研究所得修饰酶的性质。与天然酶相比，修饰酶的热稳定性及其对几种蛋白酶的抗性有所提高。将修饰酶经大鼠尾静脉静脉注射，并监测其在血浆中的循环寿命。天然酶的半衰期为50分钟，而修饰酶的半衰期约为24小时。通过对大鼠静脉注射天然酶和修饰酶来测试全身性过敏反应。对于天然酶，观察到诸如呼吸困难和翻滚等强烈反应；然而，对于修饰酶，未观察到症状或仅观察到非常微弱的反应，如抓挠。
Zotero 插件