Fleming T F, Selmair I, Schmidt H, Karch H
Department of Periodontology, Julius Maximilian University, Würzburg, Germany.
Clin Diagn Lab Immunol. 1996 Nov;3(6):678-81. doi: 10.1128/cdli.3.6.678-681.1996.
The purpose of the present study was to determine the serum immunoglobulin A (IgA), IgM, and IgG reactivities against proteins of Actinobacillus actinomycetemcomitans in patients with periodontitis. Serum samples from 20 patients with early-onset periodontitis, 20 patients with adult periodontitis, and 20 age- and sex-matched healthy controls were assessed by immunoblot analysis. IgG antibody reactivity against a sarcosyl-insoluble 110-kDa protein of A. actinomycetemcomitans was detected in 65 and 45% of patients with early-onset periodontitis and adult peritonitis, respectively, and IgA antibodies against this protein were found in 70 and 55% of these patients, respectively. However, control subjects showed no IgG reactivity, and IgA antibodies against the sarcosyl-insoluble 110-kDa protein were detected in only 5% of the patients (P < 0.05). There was no IgM antibody reactivity against this protein in any of the diseased or healthy subjects. The sensitivity and specificity of serum IgA antibody reactivity against the 110-kDa protein in detecting subgingival A. actinomycetemcomitans infection, as determined by PCR, were 77 and 66%, respectively. The results of the study indicated that the sarcosyl-insoluble 110-kDa protein is a potential candidate for use in the serodiagnosis of periodontal disease.
本研究的目的是确定牙周炎患者血清免疫球蛋白A（IgA）、IgM和IgG针对伴放线放线杆菌蛋白的反应性。通过免疫印迹分析评估了20例早发性牙周炎患者、20例成人牙周炎患者以及20例年龄和性别匹配的健康对照者的血清样本。在早发性牙周炎患者和成人牙周炎患者中，分别有65%和45%检测到针对伴放线放线杆菌肌氨酸不溶性110 kDa蛋白的IgG抗体反应性，在这些患者中，分别有70%和55%发现针对该蛋白的IgA抗体。然而，对照受试者未显示IgG反应性，仅5%的对照患者检测到针对肌氨酸不溶性110 kDa蛋白的IgA抗体（P<0.05）。在任何患病或健康受试者中均未检测到针对该蛋白的IgM抗体反应性。通过PCR测定，血清IgA抗体针对110 kDa蛋白反应性在检测龈下伴放线放线杆菌感染中的敏感性和特异性分别为77%和66%。研究结果表明，肌氨酸不溶性110 kDa蛋白是牙周疾病血清诊断的潜在候选物。
