Norwood D A, Sands J A
Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
Clin Diagn Lab Immunol. 1996 Nov;3(6):782-5. doi: 10.1128/cdli.3.6.782-785.1996.
We report the physical mapping of the toxin A and B genes to the bacterial chromosome of Clostridium difficile ATCC 43594 by pulsed-field gel electrophoresis. Single and double digestions with restriction endonucleases NruI and SacII allowed localization of the toxin genes to a specific 577-kb fragment and estimation of genome size to be approximately 3.8 megabases. This effort represents the initial step in the construction of a physical map of the whole genome.
我们通过脉冲场凝胶电泳报告了艰难梭菌ATCC 43594的毒素A和B基因在细菌染色体上的物理图谱。用限制性内切酶NruI和SacII进行单酶切和双酶切，可将毒素基因定位到一个特定的577-kb片段，并估计基因组大小约为3.8兆碱基。这项工作是构建全基因组物理图谱的第一步。
