Fluit A C, Wolfhagen M J, Verdonk G P, Jansze M, Torensma R, Verhoef J
Eijkman Winkler Laboratory for Medical Microbiology, University Hospital Utrecht, The Netherlands.
J Clin Microbiol. 1991 Nov;29(11):2666-7. doi: 10.1128/jcm.29.11.2666-2667.1991.
A total of 39 toxigenic and 20 nontoxigenic strains of Clostridium difficile were tested for the presence of either toxin A or toxin B by the polymerase chain reaction (PCR). All toxigenic strains produced cytotoxin as assayed by using highly sensitive fetal lung fibroblasts and were positive for toxin A as well as toxin B in the PCR assay. All nontoxigenic strains failed to produce toxin and were negative in the PCR assay. This study shows that nontoxigenic strains of Clostridium difficile lack the toxin A as well as the toxin B gene.
采用聚合酶链反应（PCR）对总共39株产毒艰难梭菌菌株和20株不产毒艰难梭菌菌株进行毒素A或毒素B检测。通过使用高灵敏度的胎儿肺成纤维细胞检测，所有产毒菌株均产生细胞毒素，并且在PCR检测中，毒素A和毒素B均呈阳性。所有不产毒菌株均未产生毒素，且PCR检测呈阴性。本研究表明，不产毒艰难梭菌菌株缺乏毒素A和毒素B基因。
