一种用于测定苯妥英和苯巴比妥的新型免疫分析方法的评估：一项欧洲协作对照研究的结果。

Evaluation of a new immunoassay for determination of phenytoin and phenobarbital: results of a European collaborative control study.

作者信息

Schmidt D, Goldberg V, Guelen P J, Johannessen S, Kleijn E, Meijer J W, Meinardi H, Richens A, Schneider H, Stein-Lavie Y, Symann-Louette N

出版信息

Epilepsia. 1977 Sep;18(3):367-74. doi: 10.1111/j.1528-1157.1977.tb04979.x.

DOI:10.1111/j.1528-1157.1977.tb04979.x

PMID:891490

Abstract

The performance of a new enzyme multiplied immunoassay technique (EMIT) was compared with other current methods, namely gas-liquid chromatography and thin-layer chromatography, for the determination of phenytoin and phenobarbital in serum. Forty-three serum samples were sent as unknowns to the participating laboratories for determination. The precision of repeated determinations was very similar for EMIT and chromatography. There was good agreement among gas chromatography, thin-layer chromatography, and EMIT results. Interlaboratory variability was lower for EMIT determinations of both antiepileptic drugs. The rapid analysis of small samples, made possible by the EMIT system, could have beneficial effects on the treatment of epilepsies.

摘要

为了测定血清中苯妥英和苯巴比妥的含量，将一种新的酶倍增免疫分析技术（EMIT）的性能与其他现有方法（即气液色谱法和薄层色谱法）进行了比较。43份血清样本作为未知样本送至参与实验室进行测定。EMIT和色谱法重复测定的精密度非常相似。气相色谱法、薄层色谱法和EMIT的结果之间具有良好的一致性。两种抗癫痫药物的EMIT测定的实验室间变异性较低。EMIT系统实现的小样本快速分析可能对癫痫治疗产生有益影响。