Belfield A, Duncan A M, Reavey P C
Ann Clin Biochem. 1977 Jul;14(4):218-22. doi: 10.1177/000456327701400159.
A procedure for the enzyme multiplied immunoassay of phenobarbital using the LKB Reaction Rate Analyser is described. The temperature optimum, linearity, specificity, and precision of the assay are reported. A highly significant correlation coefficient is obtained when the technique is compared with gas-liquid chromatography by analysing human sera containing a range of phenobarbital levels.
描述了一种使用LKB反应速率分析仪进行苯巴比妥酶倍增免疫测定的方法。报告了该测定的最佳温度、线性、特异性和精密度。通过分析含有一系列苯巴比妥水平的人血清,将该技术与气液色谱法进行比较时,获得了高度显著的相关系数。