Fan Y K, Croom W J, Eisen E J, Daniel L R, Black B L, McBride B W
Department of Animal Science, North Carolina State University, Raleigh 27695, USA.
J Nutr. 1996 Nov;126(11):2851-60. doi: 10.1093/jn/126.11.2851.
Five-wk-old male mice from high growth (M16) and randomly bred control (ICR) lines, plus their reciprocal crosses, ICR x M16 and M16 x ICR, were used to investigate whether whole-body O2 consumption, jejunal respiration, jejunal glucose absorption and the apparent energetic efficiency of jejunal active glucose uptake in mice are altered by genetic selection for growth as well as by heterosis and maternal effects. Whole-body O2 consumption was measured in 12 mice from each line or cross. The mice were later killed for measurement of jejunal O2, using tissue respiration chambers and jejunal glucose transport determined by 3H-3-O-methylglucose accumulation. No heterosis or maternal effects were detected in jejunal glucose active transport and active glucose uptake. Selection for growth (M16 vs. ICR) increased daily gain (1.54 vs. 1.09 g, P < 0.001), small intestinal length and weight, but did not enhance jejunal glucose transport. The apparent energetic efficiency of jejunal active glucose uptake among lines was not different (54.0, 50.4, 51.6 and 47.1 nmol ATP expended/nmol glucose uptake for M16, ICR, M16 x ICR and ICR x M16, respectively, P > 0.63). Selection for growth in mice did not result in more energetically efficient jejunal glucose absorption.
选用来自高生长品系（M16）和随机繁殖对照品系（ICR）的5周龄雄性小鼠，以及它们的正反交组合，即ICR×M16和M16×ICR，来研究小鼠的全身耗氧量、空肠呼吸、空肠葡萄糖吸收以及空肠主动摄取葡萄糖的表观能量效率是否会因生长的遗传选择以及杂种优势和母体效应而发生改变。测定了每个品系或杂交组合的12只小鼠的全身耗氧量。之后将小鼠处死，使用组织呼吸室测定空肠耗氧量，并通过3H-3-O-甲基葡萄糖积累来测定空肠葡萄糖转运。在空肠葡萄糖主动转运和主动摄取方面未检测到杂种优势或母体效应。生长选择（M16与ICR相比）增加了日增重（1.54对1.09 g，P<0.001）、小肠长度和重量，但并未增强空肠葡萄糖转运。各品系之间空肠主动摄取葡萄糖的表观能量效率并无差异（M16、ICR、M16×ICR和ICR×M16分别为54.0、50.4、51.6和47.1 nmol ATP消耗/nmol葡萄糖摄取，P>0.63）。对小鼠进行生长选择并未导致空肠葡萄糖吸收在能量利用上更高效。
