Fan Y K, Croom W J, Daniel L R, Bird A R, Black B L, Eisen E J, McBride B W
Department of Animal Science, North Carolina State University, Raleigh 27695, USA.
J Nutr. 1996 Nov;126(11):2861-6. doi: 10.1093/jn/126.11.2861.
Five-wk-old male mice from three lines were used to examine whether the apparent energetic efficiency of active jejunal glucose uptake in mouse jejunum is altered by genetic selection for different body composition. The mice lines were selected as follows: HE, high percentage of body fat with no change in body weight as a constraint; LF, low percentage of body fat; and RS, randomly bred control. Body weight was similar in all lines. Total jejunal O2 consumption and ouabain-sensitive O2 consumption were used to estimate the energy expenditure associated with glucose absorption and Na+/K(+)-ATPase activity. Tritiated 3-O-methyl-D-glucose was used to determine glucose uptake by mouse jejunum. Line LF, when compared with line HE, had lower body fat as indicated by epididymal fat pad weight (143 vs. 362 mg/mouse, P < 0.001). There were no significant differences in small intestinal weight, length and density (mg/cm) between LF and HE lines. Jejunal villus width was greater in line LF compared with line HE (115 vs. 92 microm, P < 0.002). Jejunal glucose transport and O2 consumption were not different between LF and HE lines. Ouabain-sensitive O2 consumption was not significantly different among the three lines. No differences were noted in the apparent energetic efficiency of active glucose uptake among lines.
使用来自三个品系的5周龄雄性小鼠，以研究小鼠空肠中活跃的葡萄糖摄取的表观能量效率是否会因针对不同身体组成的基因选择而改变。小鼠品系的选择如下：HE，体脂百分比高，体重无变化作为限制条件；LF，体脂百分比低；RS，随机繁殖的对照。所有品系的体重相似。使用空肠总耗氧量和哇巴因敏感耗氧量来估计与葡萄糖吸收和Na+/K(+)-ATP酶活性相关的能量消耗。用氚标记的3-O-甲基-D-葡萄糖来测定小鼠空肠的葡萄糖摄取。与HE品系相比，LF品系的体脂较低，附睾脂肪垫重量表明了这一点（143对362毫克/小鼠，P<0.001）。LF和HE品系之间的小肠重量、长度和密度（毫克/厘米）没有显著差异。与HE品系相比，LF品系的空肠绒毛宽度更大（115对92微米，P<0.002）。LF和HE品系之间的空肠葡萄糖转运和耗氧量没有差异。三个品系之间的哇巴因敏感耗氧量没有显著差异。各品系之间在活跃葡萄糖摄取的表观能量效率方面没有差异。
