Kato S, Kobayashi T, Kusuda K, Nishina Y, Nishimune Y, Yomogida K, Yamamoto M, Sakagami H, Kondo H, Ohnishi M, Chida N, Yanagawa Y, Tamura S
Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
FEBS Lett. 1996 Nov 4;396(2-3):293-7. doi: 10.1016/0014-5793(96)01119-2.
The presence of five distinct isoforms of protein phosphatase 2Cbeta (PP2Cbeta-1 approximately -5) is known. In this study, we demonstrate that the mRNA levels of PP2Cbeta-3, -4 and -5 and PP2Cbeta protein level increased during the course of the first wave of spermatogenesis in neonatal mouse testis. Northern blot and in situ hybridization analyses revealed that PP2Cbeta-3, -4 and -5 were expressed predominantly in pachytene spermatocytes and in more highly differentiated germ cells. The substrate specificity of PP2Cbeta-4 determined with artificial substrates differed from those of PP2Cbeta-3 and -5, suggesting that the difference in the structure of PP2Cbeta-3, -4 and -5 reflect their unique physiological functions in testicular germ cells.
已知存在五种不同的蛋白磷酸酶2Cβ同工型（PP2Cβ-1至-5）。在本研究中，我们证明，在新生小鼠睾丸中第一波精子发生过程中，PP2Cβ-3、-4和-5的mRNA水平以及PP2Cβ蛋白水平均升高。Northern印迹和原位杂交分析显示，PP2Cβ-3、-4和-5主要在粗线期精母细胞和分化程度更高的生殖细胞中表达。用人造底物测定的PP2Cβ-4的底物特异性不同于PP2Cβ-3和-5，这表明PP2Cβ-3、-4和-5结构上的差异反映了它们在睾丸生殖细胞中的独特生理功能。
