Marley A E, Kline A, Crabtree G, Sullivan J E, Beri R K
Cardiovascular, Musculoskeletal and Metabolism Department, Zeneca Pharmaceuticals, Cheshire, UK.
FEBS Lett. 1998 Jul 10;431(1):121-4. doi: 10.1016/s0014-5793(98)00708-x.
We have cloned a novel PP2Cbeta isoform from a human liver cDNA library which codes for a protein homologous to other mammalian PP2Cbetas at the N-terminus but with an extended C-terminus that is unique amongst the PP2Cs. The protein expressed in E. coli is indistinguishable from human recombinant PP2Calpha in its cation dependence and insensitivity to okadaic acid. Northern blot analysis of PP2Cbeta along with that of PP2Calpha shows that human PP2Cs are widely expressed and are most abundant in heart and skeletal muscle.
我们从人肝脏cDNA文库中克隆了一种新型PP2Cβ同工型，其编码的蛋白质在N端与其他哺乳动物的PP2Cβ同源，但C端延长，这在PP2C家族中是独特的。在大肠杆菌中表达的这种蛋白质在阳离子依赖性和对冈田酸的不敏感性方面与重组人PP2Cα没有区别。对PP2Cβ和PP2Cα进行Northern印迹分析表明，人PP2C广泛表达，在心脏和骨骼肌中含量最高。
