Furcht C, Eschrich K, Merte K
Department of Conservative Dentistry and Periodontology, Medical Faculty, University of Leipzig, Germany.
J Clin Periodontol. 1996 Oct;23(10):891-7. doi: 10.1111/j.1600-051x.1996.tb00508.x.
The purpose of the present investigation was to identify 2 putative periodontal pathogens: Eikenella corrodens and Actinobacillus actinomycetemcomitans by polymerase chain reaction (PCR) in vitro and in subgingival plaque. On the basis of published sequences coding for 16S rRNA two primer pairs were designed which amplify a 410 bp sequence from E. corrodens DNA and a 547 bp fragment from A. actinomycetemcomitans DNA, respectively. As few as 50 cells could be detected from pure bacterial cultures. Each of the two primer pairs was found to be specific in that it did not give any amplification product neither with cell lysates from the respective alternative bacterium nor with lysates obtained from other putative periodontal pathogens and other bacteria. The PCR method developed turned out to be a simple, rapid and reliable diagnostic tool for the detection of the target microorganisms in clinical samples.
本研究的目的是通过聚合酶链反应（PCR）在体外和龈下菌斑中鉴定两种假定的牙周病原体：腐蚀艾肯菌和伴放线放线杆菌。根据已发表的编码16S rRNA的序列，设计了两对引物，分别从腐蚀艾肯菌DNA中扩增出410 bp的序列，从伴放线放线杆菌DNA中扩增出547 bp的片段。从纯细菌培养物中最少可检测到50个细胞。发现这两对引物均具有特异性，即它们既不会从相应的替代细菌的细胞裂解物中产生任何扩增产物，也不会从其他假定的牙周病原体和其他细菌获得的裂解物中产生扩增产物。所开发的PCR方法被证明是一种用于检测临床样本中目标微生物的简单、快速且可靠的诊断工具。
