Hamilton J G, Ward R D, Dougherty M J, Maignon R, Ponce C, Ponce E, Noyes H, Zeledón R
Department of Biological Sciences, University of Keele, U.K.
Ann Trop Med Parasitol. 1996 Oct;90(5):533-41. doi: 10.1080/00034983.1996.11813079.
The terpene components of extracts prepared from male Lutzomyia longipalpis (Diptera: Psychodidae) collected from four sites in Honduras and one in Costa Rica were analysed by high-performance liquid chromatography, gas chromatography and coupled gas chromatography--mass spectrometry. The terpene components of Lu. longipalpis from other regions of South America have previously been shown to be sex pheromones. The flies from the four areas of Honduras, where Leishmania chagasi infection may lead to visceral or atypical cutaneous leishmaniasis, were found to be homogenous, all producing 9-methylgermacrene-B. Three types of terpene (9-methylgermacrene-B, a novel homosesquiterpene and a small amount of diterpene) were detected in pools of flies from Liberia, Costa Rica, where only atypical cutaneous leishmaniasis has been found. These results indicate that there are probably at least two and possibly three distinct populations of Lu. longipalpis in this region. The clinical manifestation of Leishmania chagasi infection does not appear to be dependent on which population of Lu. longipalpis transmitted the parasite.
采用高效液相色谱、气相色谱以及气相色谱 - 质谱联用技术，对从洪都拉斯的四个地点和哥斯达黎加的一个地点采集的雄性长须罗蛉（双翅目：毛蠓科）提取物中的萜类成分进行了分析。先前已证明，来自南美洲其他地区的长须罗蛉的萜类成分是性信息素。在洪都拉斯的四个地区，感染恰加斯利什曼原虫可能会导致内脏型或非典型皮肤利什曼病，发现这些地区的罗蛉是同质的，均产生9 - 甲基吉马烯 - B。在哥斯达黎加利比里亚采集的罗蛉样本中检测到三种萜类（9 - 甲基吉马烯 - B、一种新型倍半萜和少量二萜），在该地区仅发现了非典型皮肤利什曼病。这些结果表明，该地区可能至少存在两个，甚至可能三个不同的长须罗蛉种群。恰加斯利什曼原虫感染的临床表现似乎并不取决于传播该寄生虫的是哪种种群的长须罗蛉。
