牛磺酸对培养的大鼠星形胶质细胞再灌注损伤的保护作用。

Protective effect of taurine against reperfusion injury in cultured rat astrocytes.

作者信息

Matsuda T, Takuma K, Kishida Y, Azuma J, Baba A

机构信息

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.

出版信息

Adv Exp Med Biol. 1996;403:491-7. doi: 10.1007/978-1-4899-0182-8_53.

DOI:10.1007/978-1-4899-0182-8_53

PMID:8915387

Abstract

Reperfusion of cultured rat astrocytes with Ca(2+)-containing medium after exposure to Ca(2+)-free medium for a short time caused an increase in intracellular Ca2+ ([Ca2+]i), and delayed cell death (Ca2+ paradox-like injury). Exposure of astrocytes to Ca(2+)-free medium caused a marked release of taurine. Taurine (3-30 mM) reduced the reperfusion-induced increase in [Ca2+]i and attenuated the delayed glial cell death. Glycine, GABA and beta-alanine did not affect reperfusion-induced cell toxicity. The protective effect of taurine required addition at an early time during reperfusion. Ouabain and monensin mimicked reperfusion injury and their toxicity was also reduced by taurine. Taurine (3-30 mM) inhibited dose-dependently 45Ca2+ uptake stimulated by ouabain and monensin in astrocytes. These findings suggest that taurine has a protective effect against reperfusion injury via an inhibition of Na+/Ca2+ exchange activity in the reverse mode.

摘要

将培养的大鼠星形胶质细胞在短时间暴露于无钙培养基后，再用含钙培养基进行再灌注，会导致细胞内钙离子浓度（[Ca2+]i）升高，并延迟细胞死亡（类似钙反常损伤）。星形胶质细胞暴露于无钙培养基会导致牛磺酸显著释放。牛磺酸（3 - 30 mM）可减少再灌注诱导的[Ca2+]i升高，并减轻延迟性神经胶质细胞死亡。甘氨酸、γ-氨基丁酸和β-丙氨酸对再灌注诱导的细胞毒性没有影响。牛磺酸的保护作用需要在再灌注早期添加。哇巴因和莫能菌素模拟再灌注损伤，牛磺酸也可降低它们的毒性。牛磺酸（3 - 30 mM）剂量依赖性地抑制哇巴因和莫能菌素刺激的星形胶质细胞对45Ca2+的摄取。这些发现表明，牛磺酸通过抑制反向模式下的Na+/Ca2+交换活性，对再灌注损伤具有保护作用。