Ristow S S, de Avila J M, Baldwin T J, Wheeler P A, Thorgaard G H
Department of Animal Sciences, Washington State University, Pullman 99164-6332, USA.
Am J Vet Res. 1996 Nov;57(11):1576-9.
To test the immunocompetence of isogenic families of rainbow trout by measuring their ability to accept or reject skin grafts.
3 families of isogenic rainbow trout (Oncorhynchus mykiss), produced by mating homozygous females and homozygous males, plus 4 chinook salmon (O tshawytscha) were used in these experiments.
Grafts (allografts, members of the same family; autografts, donor and recipient were the same fish; and xenografts, O tshawytscha as donor) were exchanged. Grafts were applied on day 0 and removed on day 21, placed in neutral-buffered formalin, and embedded in paraffin. Lymphocytes and nuclei were counted in representative stained sections in the epidermis, dermis, and hypodermis. Results were analyzed by univariate analysis, using the Shapiro-Wilk statistic.
Autografts were retained and minimal histologic changes were apparent. Allografts were histologically similar to autografts. Xenografts were rejected.
Results indicate that the immune system of isogenic rainbow trout is unable to distinguish between family members within isogenic families, but that a vigorous response is mounted against chinook salmon xenografts. The isogenic rainbow trout are immunocompetent with respect to the phenomenon of graft rejection.
通过测量虹鳟同基因家系接受或排斥皮肤移植的能力来测试其免疫能力。
3个由纯合雌鱼和纯合雄鱼交配产生的虹鳟（Oncorhynchus mykiss）同基因家系，外加4条奇努克鲑（O tshawytscha）用于这些实验。
进行移植（同种异体移植，同一家系的成员；自体移植，供体和受体为同一条鱼；异种移植，以奇努克鲑为供体）。在第0天进行移植，第21天移除，放入中性缓冲福尔马林中，然后石蜡包埋。在表皮、真皮和皮下组织的代表性染色切片中对淋巴细胞和细胞核进行计数。结果采用单变量分析，使用夏皮罗-威尔克统计量进行分析。
自体移植得以保留，组织学变化不明显。同种异体移植在组织学上与自体移植相似。异种移植被排斥。
结果表明，虹鳟同基因家系的免疫系统无法区分同基因家系内的家庭成员，但对奇努克鲑异种移植会产生强烈反应。虹鳟在移植排斥现象方面具有免疫能力。
