Zimmerman Anastasia M, Evenhuis Jason P, Thorgaard Gary H, Ristow Sandra S
Department of Animal Sciences, Washington State University, Pullman, WA 99164-6332, USA.
Immunogenetics. 2004 Mar;55(12):825-35. doi: 10.1007/s00251-004-0645-6. Epub 2004 Feb 17.
We report the identification of a single major chromosomal region controlling natural killer (NK) cell-like activity in rainbow trout (Oncorhynchus mykiss). A genetic map based on 484 AFLP and 39 microsatellite genotypes from 106 doubled haploid fish was constructed. These fish were produced by androgenesis from a hybrid of two clonal lines divergent in NK-like activity. NK-like activities for 75 of the doubled haploids were quantified by an in vitro chromium release assay utilizing (51)Cr-labeled YAC-1 target cells. Composite interval mapping revealed a single major quantitative trait locus (QTL) associated with NK-like activity in this rainbow trout model. Genetic mapping revealed this QTL to also be unlinked to: fragmented MHC class I and MHC class II regions, the leukocyte receptor cluster, the natural killer cell enhancement factor ( NKEF) gene, the RAG-1 gene, and two QTL associated with resistance to infectious pancreatic necrosis virus in rainbow trout. Collectively, these results extend the utility of rainbow trout as an immunological model and are consistent with the idea that a single chromosomal region homologous to the natural killer cell complex (NKC) located on syntenic portions of mouse chromosome (Chr) 6, human Chr 12, and rat Chr 4 may exist in a lower vertebrate model.
我们报告了在虹鳟（Oncorhynchus mykiss）中鉴定出一个控制自然杀伤（NK）细胞样活性的单一主要染色体区域。基于106条双单倍体鱼的484个扩增片段长度多态性（AFLP）和39个微卫星基因型构建了遗传图谱。这些鱼是通过孤雄生殖从两个在NK样活性上有差异的克隆系的杂交后代中产生的。利用（51）Cr标记的YAC - 1靶细胞，通过体外铬释放试验对75个双单倍体的NK样活性进行了定量。复合区间作图揭示了在这个虹鳟模型中与NK样活性相关的一个单一主要数量性状位点（QTL）。遗传作图显示这个QTL也与以下基因不连锁：片段化的MHC I类和MHC II类区域、白细胞受体簇、自然杀伤细胞增强因子（NKEF）基因、RAG - 1基因，以及与虹鳟抗传染性胰腺坏死病毒相关的两个QTL。总体而言，这些结果扩展了虹鳟作为免疫模型的用途，并且与以下观点一致：在一个低等脊椎动物模型中可能存在一个与位于小鼠染色体（Chr）6、人类Chr 12和大鼠Chr 4的同线部分上的自然杀伤细胞复合体（NKC）同源的单一染色体区域。
