Kumar A, Singh S M
Cellular Immunology Laboratory, Department of Zoology, University of Delhi, India.
Immunol Invest. 1996 Sep-Nov;25(5-6):413-23. doi: 10.3109/08820139609055731.
In vivo growth of Dalton's lymphoma (DL), a spontaneous T cell lymphoma, has been found to increase the count of peripheral blood leukocytes (PBL). However, bone marrow cell proliferation and differentiation remained unaffected (A. Kumar and S.M. Singh, Immunol. Cell Biol., 73, 220-225). The present investigations were undertaken to study the effect of DL growth on the blastogenic response of splenocytes. A decrease in the wet weight of the spleen and in the count of splenocytes was observed at the early DL-bearing stage which, however, increased through the mid and late stages of the tumor growth. In vitro, the blastogenic response of the splenocytes of DL-bearing mice to phytohemagglutinin (PHA) was low at the early stage of the tumor, and subsequently increased at the mid and late stages. Macrophage-derived nitric oxide (NO) was found to be involved in regulating the blastogenic response of the splenocytes. DL cells are thus shown to influence splenocyte blastogenesis by altering the NO production of splenic macrophages.
已发现自发性T细胞淋巴瘤——道尔顿淋巴瘤（DL）在体内生长会使外周血白细胞（PBL）计数增加。然而，骨髓细胞的增殖和分化未受影响（A. 库马尔和S.M. 辛格，《免疫与细胞生物学》，73卷，220 - 225页）。开展本研究以探讨DL生长对脾细胞增殖反应的影响。在荷瘤早期观察到脾脏湿重和脾细胞计数下降，但在肿瘤生长的中晚期增加。在体外，荷瘤小鼠脾细胞对植物血凝素（PHA）的增殖反应在肿瘤早期较低，随后在中晚期增加。发现巨噬细胞衍生的一氧化氮（NO）参与调节脾细胞的增殖反应。因此表明DL细胞通过改变脾巨噬细胞的NO产生来影响脾细胞增殖。
