Khare V, Singh S M, Sodhi A
School of Biotechnology, Banaras Hindu University, Varanasi, India.
Nat Immun. 1998;16(5-6):215-27. doi: 10.1159/000069448.
The present investigation was carried out to study the effect of tumor growth on the spleen of an aged host. Dalton's lymphoma (DL), a spontaneous T cell lymphoma, was grown in mice of different age groups classified as young, adult or old on the basis of their reproductive status. Splenocytes obtained from normal and tumor-bearing young, adult and old mice were checked for an in vitro blastogenic response to concanavalin A (Con A), colony-forming ability and apoptosis. There was an enhanced apoptosis of splenocytes and a concomitant inhibition of splenocyte blastogenesis and their responsiveness to the mitogenic stimulus of Con A in aged mice. The counts of granulocyte macrophage- and macrophage-colony forming units were significantly enhanced in the spleen of tumor-bearing adult mice. It is proposed that the DL-growth-dependent increase in the size of the spleen in adult mice is due to an increased blastogenesis of splenocytes, which, however, may not be applicable in the case of old tumor-bearing mice. The role of splenic macrophages in the regulation of the functions of the spleen by macrophage-derived NO is shown.
本研究旨在探讨肿瘤生长对老年宿主脾脏的影响。道尔顿淋巴瘤（DL）是一种自发性T细胞淋巴瘤，在根据生殖状态分为年轻、成年或老年的不同年龄组小鼠体内生长。从正常以及荷瘤的年轻、成年和老年小鼠中获取脾细胞，检测其对刀豆球蛋白A（Con A）的体外增殖反应、集落形成能力和凋亡情况。老年小鼠脾细胞凋亡增强，同时脾细胞增殖以及对Con A促有丝分裂刺激的反应性受到抑制。荷瘤成年小鼠脾脏中粒细胞巨噬细胞集落形成单位和巨噬细胞集落形成单位的数量显著增加。研究表明，成年小鼠中DL生长依赖的脾脏大小增加是由于脾细胞增殖增加，但这可能不适用于老年荷瘤小鼠。研究显示了脾巨噬细胞在巨噬细胞衍生的一氧化氮对脾脏功能调节中的作用。
