Ochoa M T, Valderrama L, Ochoa A, Zea A, Escobar C E, Moreno L H, Falabella R
Dermatology Service, Universidad del Valle, Cali, Colombia.
Int J Dermatol. 1996 Nov;35(11):786-90. doi: 10.1111/j.1365-4362.1996.tb02974.x.
This study analyzes the major clinical characteristics of patients with active leprosy in relation to the in vitro immune response to the T-lymphocyte activator anti-CD3.
Thirty-eight patients with an established diagnosis of leprosy were classified according to the Ridley and Jopling table. Peripheral blood mononuclear cells from both lepromatous leprosy (LL) and tuberculoid leprosy (TL) patients and healthy controls were used to evaluate lymphocyte proliferation; immunoenzymatic assays were used to evaluate cytokine production (IL-1, IL-2, IL-4, IL-6, IL-10, IFN-gamma).
Peripheral blood mononuclear cells from both LL and TL patients displayed blastogenic responses to anti-CD3. The cytokines IL-1 beta, IL-6, IL-10, and IFN-gamma were detected in culture supernatants. Endogenous production of IL-1 beta was significantly higher in cell cultures from patients with the lepromatous form of the disease compared to those with tuberculoid leprosy. Production of IL-6 in response to anti-CD3 was observed in a significantly higher proportion of LL than TL patients (P = 0.0025). Gamma-interferon production did not differ between TL and LL, but a direct correlation was observed between time of multidrug treatment and IFN production in vitro (P = 0.016). Interleukin-10 was detected in culture supernatants of lymphocytes activated by anti-CD3 from both patient groups, but not from healthy controls.
The findings of this study suggest that patients with the two distinct forms of leprosy are capable of responding to a polyclonal T-lymphocyte stimulus such as anti-CD3 and provide evidence suggestive of alterations in the immune responses mediated by cytokines that may contribute to the spectrum of disease and response to treatment.
本研究分析活动性麻风患者的主要临床特征与对T淋巴细胞激活剂抗CD3的体外免疫反应之间的关系。
38例确诊麻风患者根据里德利和乔普林分类表进行分类。使用瘤型麻风（LL）和结核样型麻风（TL）患者及健康对照者的外周血单个核细胞评估淋巴细胞增殖；采用免疫酶测定法评估细胞因子产生情况（白细胞介素-1、白细胞介素-2、白细胞介素-4、白细胞介素-6、白细胞介素-10、γ干扰素）。
LL和TL患者的外周血单个核细胞均对抗CD3表现出增殖反应。培养上清液中检测到细胞因子白细胞介素-1β、白细胞介素-6、白细胞介素-10和γ干扰素。与结核样型麻风患者相比，瘤型麻风患者细胞培养物中白细胞介素-1β的内源性产生显著更高。LL患者中对抗CD3产生白细胞介素-6的比例显著高于TL患者（P = 0.0025）。TL和LL患者之间γ干扰素产生无差异，但观察到多药治疗时间与体外干扰素产生之间存在直接相关性（P = 0.016）。两组患者经抗CD3激活的淋巴细胞培养上清液中均检测到白细胞介素-10，但健康对照者中未检测到。
本研究结果表明，两种不同类型麻风患者能够对多克隆T淋巴细胞刺激如抗CD3作出反应，并提供证据表明细胞因子介导的免疫反应发生改变，这可能有助于疾病谱和对治疗的反应。
