Zea A H, Ochoa M T, Ghosh P, Longo D L, Alvord W G, Valderrama L, Falabella R, Harvey L K, Saravia N, Moreno L H, Ochoa A C
Immunotherapy Program, Stanley S. Scott Cancer Center, Louisiana State University Medical Center, New Orleans 70112, USA.
Infect Immun. 1998 Feb;66(2):499-504. doi: 10.1128/IAI.66.2.499-504.1998.
Advanced stages of mycobacterial diseases such as leprosy and tuberculosis are characterized by a loss of T-cell function. The basis of this T-cell dysfunction is not well understood. The present report demonstrates major alterations in the expression of signal transduction molecules in T cells of leprosy patients. These alterations were most frequently observed in lepromatous leprosy (LL) patients. Of 29 LL patients, 69% had decreased T-cell receptor zeta-chain expression, 48% had decreased p56(lck) tyrosine kinase, and 63% had a loss of nuclear transcription factor NF-kappaB p65. An electrophoretic mobility shift assay with the gamma interferon core promoter region revealed a loss of the Th1 DNA-binding pattern in LL patients. In contrast, tuberculoid leprosy patients had only minor signal transduction alterations. These novel findings might improve our understanding of the T-cell dysfunction observed in leprosy and other infectious diseases and consequently might lead to better immunologic evaluation of patients.
麻风病和结核病等分枝杆菌病的晚期特征是T细胞功能丧失。这种T细胞功能障碍的基础尚不清楚。本报告显示了麻风病患者T细胞中信号转导分子表达的主要变化。这些变化最常出现在瘤型麻风（LL）患者中。在29例LL患者中，69%的患者T细胞受体ζ链表达降低，48%的患者p56(lck)酪氨酸激酶降低，63%的患者核转录因子NF-κB p65缺失。用γ干扰素核心启动子区域进行的电泳迁移率变动分析显示LL患者中Th1 DNA结合模式缺失。相比之下，结核样型麻风患者只有轻微的信号转导改变。这些新发现可能会增进我们对麻风病和其他传染病中观察到的T细胞功能障碍的理解，从而可能导致对患者进行更好的免疫学评估。
