Lin J A, Shyu C L, Yamaguchi T, Takagi M
Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan, ROC.
J Vet Med Sci. 1996 Oct;58(10):1007-9. doi: 10.1292/jvms.58.10_1007.
Two strains (Strains 39 & B) of Haemophilus paragallinarum were isolated and identified as serotype C by dot-blotting analysis in Taiwan in 1994. To identify their virulence and pathogenicity in Taiwanese local chickens, local chickens were inoculated via nostril with the isolates, and examined for 14 days. Clinical signs of swollen face, and nasal discharge were first seen at 24 hr after inoculation, and the infectivity of Strains 39 and B at 14 days after inoculation among the inoculated chickens was 50.0-71.4% and 55.6-77.8%, respectively. From the results, we concluded that the two bacterial isolates were pathogenic to local chickens with a maximum morbidity of 77.8%.
1994年在台湾分离出两株副鸡嗜血杆菌（菌株39和B），通过斑点印迹分析鉴定为C血清型。为了确定它们对台湾本地鸡的毒力和致病性，将分离株经鼻孔接种到本地鸡中，并观察14天。接种后24小时首次出现脸部肿胀和鼻液等临床症状，接种后14天，接种鸡中菌株39和B的感染率分别为50.0% - 71.4%和55.6% - 77.8%。根据结果，我们得出结论，这两株细菌分离株对本地鸡具有致病性，最高发病率为77.8%。
