Terzolo H R, Paolicchi F A, Sandoval V E, Blackall P J, Yamaguchi T, Iritani Y
Convenio CERBAS-LAPSA, Instituto Nacional de Tecnología Agropecuaria, Departamento de Producción Animal, Balcarce, Argentina.
Avian Dis. 1993 Apr-Jun;37(2):310-4.
The biochemical and serological properties of Haemophilus paragallinarum isolates recovered from 11 recent outbreaks of infectious coryza in layer hens and one case of swollen-head syndrome in broilers in Argentina are described. Twenty-four isolates had the typical biochemical properties of H. paragallinarum. All isolates were serotyped according to the Page scheme. Ten of the isolates were serovar A, 11 were serovar B, one was serovar C, and two isolates could not be serotyped. The isolates were also examined using a panel of monoclonal antibodies (MAbs) for Page serovars A (one MAb available) and C (three MAbs available). The serovar B isolates all failed to react with any MAb. The serovar C isolate reacted with all three serovar C MAbs but not with the serovar A MAb. Only six of the 10 serovar A isolates reacted with the serovar A MAb. These results indicate that H. paragallinarum isolates from Argentina are antigenically distinct from those examined in other countries, and it is suggested that coryza vaccines intended for use in Argentina may be more effective if based on local strains.
描述了从阿根廷近期11起蛋鸡传染性鼻炎疫情和1起肉鸡肿头综合征病例中分离出的副鸡嗜血杆菌菌株的生化和血清学特性。24株分离株具有副鸡嗜血杆菌的典型生化特性。所有分离株均按照佩奇分型方案进行血清分型。其中10株为A血清型，11株为B血清型，1株为C血清型，2株无法进行血清分型。还使用一组针对佩奇A血清型（有1种单克隆抗体）和C血清型（有3种单克隆抗体）的单克隆抗体对分离株进行检测。B血清型分离株均未与任何单克隆抗体发生反应。C血清型分离株与所有3种C血清型单克隆抗体发生反应，但未与A血清型单克隆抗体发生反应。10株A血清型分离株中只有6株与A血清型单克隆抗体发生反应。这些结果表明，来自阿根廷的副鸡嗜血杆菌分离株在抗原性上与其他国家检测的分离株不同，建议用于阿根廷的鼻炎疫苗若基于当地菌株可能会更有效。
